Journal: Dentistry Journal

Article Title: In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

doi: 10.3390/dj12030076

Figure Lengend Snippet: Representative histological images of (H&E) ( A , D , G , J ), Masson’s trichrome ( B , E , H , K ) and osteocalcin immune staining ( C , F , I , L ) are shown at 4 weeks post-treatment. Coronal sections through the mid-point of the defects were prepared from decalcified specimens. Fibrous connective tissues were seen to be mostly filled in the defect areas in the control group ( A – C ). The group treated with SA hydrogel as the only grafted material showing fibrous and adipose tissue ( D – F ). lacuna spaces were observed in the newly formed lamellar bone in NHH with a wide bone marrow space ( G – I ). DDMH-treated groups showed considerable new bone trabeculae formation ( J – L ). BT: bone trabeculae; FT: fibrous tissue. Arrows represent MT- and OCN-positive reactions. Scale bar: 100 μm.

Article Snippet: Subsequently, the samples were embedded in paraffin; and after staining with hematoxylin and eosin (H&E), Masson’s trichrome (MT) (Cat# HT15, Sigma Aldrich), and immunohistochemical staining with a polyclonal anti-rabbit osteocalcin antibody (OCN; Cat# GB11233, diagnostic biosystems) at a 1:150 dilution, histological examination was conducted using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan).

Techniques: Staining, Control

Journal: Dentistry Journal

Article Title: In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

doi: 10.3390/dj12030076

Figure Lengend Snippet: Representative histological images of (H&E) ( A , D , G , J ), Masson’s trichrome ( B , E , H , K ) and osteocalcin immune staining ( C , F , I , L ) are shown at 8 weeks post-treatment. Rather than the newly created bone, the defect locations in the control group were found to be primarily filled with fibrous connective tissues and adipose tissue ( A – C ). Circular new bony islands with the presence of early woven bone were observed in the group treated with SA as the only grafted material ( D – F ). Newly formed lamellar bone was observed in NHH with a wide bone marrow space ( G – I ). In contrast, DDMH-treated groups showed considerable new bone formation at 8 weeks with relatively narrow marrow spaces ( J – L ). BT means bone trabeculae; FT: fibrous tissue. Arrows represent MT- and OCN-positive reactions. Scale bar: 100 μm.

Article Snippet: Subsequently, the samples were embedded in paraffin; and after staining with hematoxylin and eosin (H&E), Masson’s trichrome (MT) (Cat# HT15, Sigma Aldrich), and immunohistochemical staining with a polyclonal anti-rabbit osteocalcin antibody (OCN; Cat# GB11233, diagnostic biosystems) at a 1:150 dilution, histological examination was conducted using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan).

Techniques: Staining, Control

Journal: Stem Cell Research & Therapy

Article Title: Efficient bone regeneration of BMP9-stimulated human periodontal ligament stem cells (hPDLSCs) in decellularized bone matrix (DBM) constructs to model maxillofacial intrabony defect repair

doi: 10.1186/s13287-022-03221-3

Figure Lengend Snippet: Immunohistochemical staining of the newly formed tissues with different cell concentrations and distinct BMP9 MOIs. A Representative immunohistochemical staining images of CD31 and B α-SMA. The newly formed blood vessels are indicated with arrows. Scale bar = 50 μm

Article Snippet: Rabbit anti-OCN (Servicebio, GB11233, 1:200), Rabbit anti-CD31 (Servicebio, GB113151, 1:600) antibody and rabbit anti-α-SMA (Servicebio, GB111364, 1:1000) antibody were used as the primary antibodies.

Techniques: Immunohistochemical staining, Staining

Journal: Stem Cell Research & Therapy

Article Title: Efficient bone regeneration of BMP9-stimulated human periodontal ligament stem cells (hPDLSCs) in decellularized bone matrix (DBM) constructs to model maxillofacial intrabony defect repair

doi: 10.1186/s13287-022-03221-3

Figure Lengend Snippet: Immunohistochemical staining of the newly formed tissues with different cell concentrations and distinct BMP9 MOIs. A Representative immunohistochemical staining images of CD31 and B α-SMA. The newly formed blood vessels are indicated with arrows. Scale bar = 50 μm

Article Snippet: Rabbit anti-OCN (Servicebio, GB11233, 1:200), Rabbit anti-CD31 (Servicebio, GB113151, 1:600) antibody and rabbit anti-α-SMA (Servicebio, GB111364, 1:1000) antibody were used as the primary antibodies.

Techniques: Immunohistochemical staining, Staining

Journal: Bioactive Materials

Article Title: Throw out an oligopeptide to catch a protein: Deep learning and natural language processing-screened tripeptide PSP promotes Osteolectin-mediated vascularized bone regeneration

doi: 10.1016/j.bioactmat.2024.11.011

Figure Lengend Snippet: DL and NLP-screened tripeptide PSP promoted vascularized bone regeneration in critical-sized cranial defect model . (A) Illustration of the experimental procedure; (B) Representative SEM images of GelMA, PSP-GelMA and KLT-GelMA hydrogels (scale bar, 20 μm); Micro-CT reconstruction from top view, coronal view and sagittal view 4-weeks (C) and 8-weeks (D) after implantation (scale bar, 1 mm); (E) Semi-quantitative analysis of Micro-CT; Representative images of HE staining 4-weeks (F) and 8-weeks (G) after implantation (scale bar, low magnification 500 μm/high magnification 100 μm); Representative images of Masson's trichrome staining 4-weeks (H) and 8-weeks (I) after implantation (scale bar, low magnification 500 μm/high magnification 100 μm); Representative images of immunofluorescence staining of CD31 and EMCN 4-weeks (J) and 8-weeks (K) after implantation (DAPI in blue, CD31 in red, EMCN in green, scale bar, low magnification 500 μm/high magnification 50 μm, arrow indicated CD31 and EMCN co-labeled type H vessels). Data are presented as means ± SD. Significant difference with respect to the control groups (∗), ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Primary mouse anti-CD31 antibody (1:200, 66065-2-lg, Proteintech, USA), primary rabbit anti-EMCN antibody (1:200, YT1557, Immunoway, USA) and primary rabbit anti-OCN antibody (1:200, GB11233, Servicebio, China) were used for immunofluorescence (IF) and immunohistochemistry (IHC) staining.

Techniques: Micro-CT, Staining, Immunofluorescence, Labeling, Control

Journal: Bioactive Materials

Article Title: Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair

doi: 10.1016/j.bioactmat.2023.02.013

Figure Lengend Snippet: (A) Histological analysis of subcutaneous implantation constructs for nervous structures. Blue, red, and green signals represent the nucleus, MBP, and NF200, respectively. Scale bar = 200 or 50 μm. (B) Histological analysis of subcutaneous implantation constructs for vascular structures. The blue, red, and green signals represent the nucleus, α-SMA, and CD31, respectively. Scale bar = 200 or 50 μm. (C, D) After 7 and 14 d of implantation, ultrasound images of implanted structures were obtained. White asterisks and arrowheads denote the hydrogel and vascular systems, respectively. Scale bar = 2 mm. (E) Scratch wound assay of EPCs. Scale bar = 200 μm. (F) Tube formation assay of EPCs. Scale bar = 100 μm. (G) Analysis of the migration assay. (H) Quantitative analysis of (h1) branching points and (h2) total tube length. (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: For immunofluorescence, OCN (1:200, GB11233, Servicebio, Wuhan, China), CD31 (1:200, GB12063, Servicebio, Wuhan, China), α-SMA (1:200, GB111364, Servicebio, Wuhan, China), NF200 (1:500, GB12144, Servicebio, Wuhan, China), or MBP antibodies (1:200, GB11226, Servicebio, Wuhan, China) were incubated overnight at 4 °C.

Techniques: Construct, Scratch Wound Assay Assay, Tube Formation Assay, Migration